Makonda mwatsatanetsatane bulaketi zitsulo zosapanga dzimbiri zitsulo zopondaponda mbali

Njira ya electrophoresis imaphatikizapo njira zotsatirazi:

1. Kukonzekera kwachitsanzo: Sankhani njira yoyenera yochotsera chitsanzo ndikuchita zofunikira zowonetseratu molingana ndi mtundu wa chitsanzo, monga kusokonezeka kwa selo, mapuloteni a solubilization, ndi zina zotero.
2. Kukonzekera kwa buffer: Buffer imathandizira kuti pH ikhale yosasunthika, ikupereka ma ionic conductivity ndi zitsanzo za diluting panthawi yoyesera electrophoresis. Pokonzekera ma buffers, ma reagents ayenera kuyezedwa molondola ndikuchepetsedwa moyenera.
3. Kukonzekera kwa thanki ya electrophoresis ndi ma electrodes: Sambani thanki ya electrophoresis ndi ma electrode kuti muwonetsetse kuti palibe zonyansa. Onetsetsani kuti maelekitirodi agwirizana kwambiri ndi makoma a thanki komanso kuti mizere yolumikizira sinathyoke kapena yofupikitsidwa.
4. Konzani utoto: Sungunulani utoto wa electrophoretic m'madzi kuti mupange yunifolomu ndi kuyimitsidwa kokhazikika. Zomwe zimapangidwira ziyenera kusankhidwa malinga ndi zofunikira za workpiece ndi malo ogwiritsira ntchito, ndipo nthawi zambiri zimaphatikizapo utomoni, zosungunulira, zochizira, zodzaza, ndi zina zotero.
5. Konzani tanki ya electrophoresis: Ikani tanki ya electrophoresis yomwe ili ndi utoto mu zipangizo za electrophoresis, ndikugwirizanitsa ma electrode abwino ndi oipa ku magetsi. Elekitirodi yabwino imalumikizidwa ndi mbale yachitsulo mu thanki ya electrophoresis, ndipo electrode yoyipa imalumikizidwa ndi workpiece kuti ipange gawo lamagetsi pansi pamagetsi.
6. Sinthani magawo a penti: Malingana ndi zofunikira za workpiece ndi utoto, sinthani magawo a utoto mu thanki ya electrophoresis, monga ndende, pH mtengo, kutentha ndi magetsi, etc. Kusintha kwa magawowa kungadziwike kupyolera mu mayesero ndi mayesero.
7. Kuyika kwachitsanzo: Onjezani chinthu kuti chiyesedwe mu thanki ya electrophoresis yokonzedwa kuti isiyanitse. Mukatsegula zitsanzo, chisamaliro chiyenera kutengedwa kuti mpweya usalowe mu gel osakaniza kuti usawononge zotsatira zolekanitsa. Dziwani kuchuluka koyenera kokweza kutengera kukula kwachitsanzo ndi kukhazikika. Kuchuluka kapena kucheperako kungakhudze zotsatira zoyeserera.
8. Yambitsani electrophoresis: Ikani chogwiritsira ntchito chogwiritsidwa ntchito mu thanki ya electrophoresis ndikuonetsetsa kuti chogwiritsira ntchito chikugwirizana ndi utoto. Yatsani mphamvu, kuchititsa kuti tinthu tating'onoting'ono kapena ayoni mupenti asunthike ndikuyika pamwamba pa chogwiriracho pansi pamagetsi.
9. Kuchiza kuchiritsa: Pambuyo popaka pamwamba pa workpiece kufika pa makulidwe ofunikira, tengani chogwirira ntchito kuchokera mu tank electrophoresis ndikuchitumiza ku uvuni kapena mpweya wouma kuti ukhale wolimba.

Zomwe zili pamwambazi ndizotsatira zonse za ndondomeko ya electrophoresis. Zochita zenizeni zitha kusinthidwa chifukwa cha zida ndi njira zosiyanasiyana. Panthawi yonse ya electrophoresis, magawo osiyanasiyana ndi njira zogwirira ntchito ziyenera kuyang'aniridwa mosamalitsa kuti zitsimikizire kuti zinthu zili bwino komanso kukhazikika.